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Solid phase chemistry to covalently and reversibly capture thiolated RNA
Here, we describe an approach to enrich newly transcribed RNAs from primary mouse neurons using 4-thiouridine (s(4)U) metabolic labeling and solid phase chemistry. This one-step enrichment procedure captures s(4)U-RNA by using highly efficient methane thiosulfonate (MTS) chemistry in an immobilized...
में बचाया:
| में प्रकाशित: | Nucleic Acids Res |
|---|---|
| मुख्य लेखकों: | , , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
Oxford University Press
2018
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6101502/ https://ncbi.nlm.nih.gov/pubmed/29986098 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky556 |
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