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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Cyclin-dependent kinase 1 (Cdk1) is a master controller for the cell cycle in all eukaryotes and phosphorylates an estimated 8 - 13% of the proteome; however, the number of identified targets for Cdk1, particularly in human cells is still low. The identification of Cdk1-specific phosphorylation site...

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Bibliografische gegevens
Gepubliceerd in:J Vis Exp
Hoofdauteurs: Cui, Heying, Loftus, Kyle M., Noell, Crystal R., Solmaz, Sozanne R.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MyJove Corporation 2018
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101106/
https://ncbi.nlm.nih.gov/pubmed/29782014
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57674
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