A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

The clustered regularly interspersed palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) prokaryotic adaptive immune defense system has been co-opted as a powerful tool for precise eukaryotic genome engineering. Here, we present a rapid and simple method using chimeric single guide RNAs...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Prior, Harriet, MacConnachie, Lauren, Martinez, Jose L., Nicholl, Georgina C.B., Beg, Asim A.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2018
Fag:
Genetics
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101052/
https://ncbi.nlm.nih.gov/pubmed/29757293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57518
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