Monolith affinity chromatography for the rapid quantification of a single‐chain variable fragment immunotoxin

We developed a novel analytical method for concentration determination of tandem single‐chain antibody diphtheria toxin (immunotoxin). The method is based on polymethacrylate monoliths with Protein L ligands as the binding moiety. Different buffers were tested for elution of the Protein L‐bound immu...

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Bibliografiset tiedot
Julkaisussa:J Sep Sci
Päätekijät: Satzer, Peter, Sommer, Ralf, Paulsson, Johanna, Rodler, Agnes, Zehetner, Romana, Hofstädter, Klaus, Klade, Christoph, Jungbauer, Alois
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: John Wiley and Sons Inc. 2018
Aiheet:
Liquid Chromatography
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC6099420/
https://ncbi.nlm.nih.gov/pubmed/29873445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jssc.201800257
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