Identification and removal of sequencing artifacts produced by mispriming during reverse transcription in multiple RNA-seq technologies

The quality of RNA sequencing data relies on specific priming by the primer used for reverse transcription (RT-primer). Nonspecific annealing of the RT-primer to the RNA template can generate reads with incorrect cDNA ends and can cause misinterpretation of data (RT mispriming). This kind of artifac...

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Bibliographic Details
Published in:RNA
Main Authors: Shivram, Haridha, Iyer, Vishwanath R.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2018
Subjects:
Method
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6097653/
https://ncbi.nlm.nih.gov/pubmed/29950518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.066217.118
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