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i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats

Zygote-microinjection or in vitro electroporation of isolated zygotes are now widely used methods to produce genome-edited mice. However, these technologies require laborious and time-consuming ex vivo handling of fertilized eggs, including zygote isolation, gene delivery into zygotes and embryo tra...

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Bibliografiska uppgifter
I publikationen:Sci Rep
Huvudupphovsmän: Takabayashi, Shuji, Aoshima, Takuya, Kabashima, Katsuya, Aoto, Kazushi, Ohtsuka, Masato, Sato, Masahiro
Materialtyp: Artigo
Språk:Inglês
Publicerad: Nature Publishing Group UK 2018
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6089882/
https://ncbi.nlm.nih.gov/pubmed/30104681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-30137-x
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