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Kif15 functions as an active mechanical ratchet

Kif15 is a kinesin-12 that contributes critically to bipolar spindle assembly in humans. Here we use force-ramp experiments in an optical trap to probe the mechanics of single Kif15 molecules under hindering or assisting loads and in a variety of nucleotide states. While unloaded Kif15 is establishe...

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Détails bibliographiques
Publié dans:Mol Biol Cell
Auteurs principaux: McHugh, Toni, Drechsler, Hauke, McAinsh, Andrew D., Carter, Nicolas J., Cross, Robert A.
Format: Artigo
Langue:Inglês
Publié: The American Society for Cell Biology 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6080711/
https://ncbi.nlm.nih.gov/pubmed/29771628
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E18-03-0151
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