Comprehensive multi-center assessment of accuracy, reproducibility and bias of small RNA-seq methods for quantitative miRNA profiling

RNA-seq is increasingly employed for quantitative profiling of small RNAs (e.g., microRNAs, piRNAs, snoRNAs) in diverse sample types including isolated cells, tissues and cell-free biofluids. The accuracy and reproducibility of the multiple small RNA-seq library preparation methods in use, however,...

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Publicado no:Nat Biotechnol
Main Authors: Giraldez, MD, Spengler, RM, Etheridge, A, Godoy, PM, Barczak, AJ, Srinivasan, S, De Hoff, PL, Tanriverdi, K, Courtright, A, Lu, S, Khoory, J, Rubio, R, Baxter, D, Driedonks, TAP, Buermans, HPJ, Nolte-‘t Hoen, ENM, Jiang, H, Wang, K, Ghiran, I, Wang, Y, Van Keuren-Jensen, K, Freedman, JE, Woodruff, PG, Laurent, LC, Erle, DJ, Galas, DJ, Tewari, M
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6078798/
https://ncbi.nlm.nih.gov/pubmed/30010675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.4183
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