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Technical approaches to reduce interference of Fetal calf serum derived RNA in the analysis of extracellular vesicle RNA from cultured cells

Foetal calf serum (FCS) is a common supplement of cell culture medium and a known source of contaminating extracellular vesicles (EV) containing RNA. Because of a high degree of sequence similarity among homologous non-coding RNAs of mammalian species, residual FCS-RNA in culture medium may interfer...

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Detalhes bibliográficos
Publicado no:J Extracell Vesicles
Main Authors: Driedonks, Tom A. P., Nijen Twilhaar, Maarten K., Nolte-‘t Hoen, Esther N. M.
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6292350/
https://ncbi.nlm.nih.gov/pubmed/30559953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/20013078.2018.1552059
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