Efficient homology-directed gene editing by CRISPR/Cas9 in human stem and primary cells using tube electroporation

CRISPR/Cas9 efficiently generates gene knock-out via nonhomologous end joining (NHEJ), but the efficiency of precise homology-directed repair (HDR) is substantially lower, especially in the hard-to-transfect human stem cells and primary cells. Herein we report a tube electroporation method that can...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Xu, Xiaoyun, Gao, Dongbing, Wang, Ping, Chen, Jian, Ruan, Jinxue, Xu, Jie, Xia, Xiaofeng
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6076306/
https://ncbi.nlm.nih.gov/pubmed/30076383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-30227-w
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