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Efficient homology-directed gene editing by CRISPR/Cas9 in human stem and primary cells using tube electroporation
CRISPR/Cas9 efficiently generates gene knock-out via nonhomologous end joining (NHEJ), but the efficiency of precise homology-directed repair (HDR) is substantially lower, especially in the hard-to-transfect human stem cells and primary cells. Herein we report a tube electroporation method that can...
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| Veröffentlicht in: | Sci Rep |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group UK
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6076306/ https://ncbi.nlm.nih.gov/pubmed/30076383 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-30227-w |
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