Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

The Cas9/guide RNA (Cas9/gRNA) system is commonly used for genome editing. mRNA expressing Cas9 can induce innate immune responses, reducing Cas9 expression. First-generation Cas9 mRNAs were modified with pseudouridine and 5-methylcytosine to reduce innate immune responses. We combined four approach...

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Bibliographic Details
Published in:Mol Ther Nucleic Acids
Main Authors: Vaidyanathan, Sriram, Azizian, Krist T., Haque, A.K.M. Ashiqul, Henderson, Jordana M., Hendel, Ayal, Shore, Sabrina, Antony, Justin S., Hogrefe, Richard I., Kormann, Michael S.D., Porteus, Matthew H., McCaffrey, Anton P.
Format: Artigo
Language:Inglês
Published: American Society of Gene & Cell Therapy 2018
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6076213/
https://ncbi.nlm.nih.gov/pubmed/30195789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtn.2018.06.010
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