Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

Cas9-mediated gene editing is a powerful tool for addressing research questions in arthropods. Current approaches rely upon delivering Cas9 ribonucleoprotein (RNP) complex by embryonic microinjection, which is challenging, is limited to a small number of species, and is inefficient even in optimized...

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Udgivet i:Nat Commun
Main Authors: Chaverra-Rodriguez, Duverney, Macias, Vanessa M., Hughes, Grant L., Pujhari, Sujit, Suzuki, Yasutsugu, Peterson, David R., Kim, Donghun, McKeand, Sage, Rasgon, Jason L.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6070532/
https://ncbi.nlm.nih.gov/pubmed/30068905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-05425-9
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