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Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis elegans

Green fluorescent protein (GFP) is widely used as a molecular tool to assess protein expression and localization. In C. elegans, the signal from weakly expressed GFP fusion proteins is masked by autofluorescence emitted from the intestinal lysosome-related gut granules. For instance, the GFP fluores...

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Bibliografski detalji
Izdano u:Bio Protoc
Glavni autori: Teuscher, Alina C., Ewald, Collin Y.
Format: Artigo
Jezik:Inglês
Izdano: 2018
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6067662/
https://ncbi.nlm.nih.gov/pubmed/30073182
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2940
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