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Validating Antibodies for Quantitative Western Blot Measurements with Microwestern Array

Fluorescence-based western blots are quantitative in principal, but require determining linear range for each antibody. Here, we use microwestern array to rapidly evaluate suitable conditions for quantitative western blotting, with up to 192 antibody/dilution/replicate combinations on a single stand...

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Podrobná bibliografie
Vydáno v:Sci Rep
Hlavní autoři: Koch, Rick J., Barrette, Anne Marie, Stern, Alan D., Hu, Bin, Bouhaddou, Mehdi, Azeloglu, Evren U., Iyengar, Ravi, Birtwistle, Marc R.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6063895/
https://ncbi.nlm.nih.gov/pubmed/30054510
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-29436-0
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