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A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae

Homologous recombination faithfully restores the sequence information interrupted by a DNA double-strand break by referencing an intact DNA molecule as a template for repair DNA synthesis. DNA synthesis is primed from 3′-OH end of the invading DNA strand in the displacement loop (D-loop). Here, we d...

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Publicat a:Methods Enzymol
Autors principals: Piazza, Aurèle, Koszul, Romain, Heyer, Wolf-Dietrich
Format: Artigo
Idioma:Inglês
Publicat: 2018
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6063363/
https://ncbi.nlm.nih.gov/pubmed/29523235
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2017.11.024
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