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Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 Regulation in Naive Pluripotency Maintenance and Dissolution

The genetic basis of naive pluripotency maintenance and loss is a central question in embryonic stem cell biology. Here, we deploy CRISPR-knockout-based screens in mouse embryonic stem cells to interrogate this question through a genome-wide, non-biased approach using the Rex1GFP reporter as a pheno...

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Detalhes bibliográficos
Publicado no:Cell Rep
Main Authors: Li, Meng, Yu, Jason S.L., Tilgner, Katarzyna, Ong, Swee Hoe, Koike-Yusa, Hiroko, Yusa, Kosuke
Formato: Artigo
Idioma:Inglês
Publicado em: Cell Press 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6057492/
https://ncbi.nlm.nih.gov/pubmed/29996108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2018.06.027
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