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PBMC fixation and processing for Chromium single-cell RNA sequencing

BACKGROUND: Interest in single-cell transcriptomic analysis is growing rapidly, especially for profiling rare or heterogeneous populations of cells. In almost all reported works investigators have used live cells, which introduces cell stress during preparation and hinders complex study designs. Rec...

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Detalhes bibliográficos
Publicado no:J Transl Med
Main Authors: Chen, Jinguo, Cheung, Foo, Shi, Rongye, Zhou, Huizhi, Lu, Wenrui
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6050658/
https://ncbi.nlm.nih.gov/pubmed/30016977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12967-018-1578-4
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