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Structural and biochemical analysis of the dual-specificity Trm10 enzyme from Thermococcus kodakaraensis prompts reconsideration of its catalytic mechanism

tRNA molecules get heavily modified post-transcriptionally. The N-1 methylation of purines at position 9 of eukaryal and archaeal tRNA is catalyzed by the SPOUT methyltranferase Trm10. Remarkably, while certain Trm10 orthologs are specific for either guanosine or adenosine, others show a dual specif...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Singh, Ranjan Kumar, Feller, André, Roovers, Martine, Van Elder, Dany, Wauters, Lina, Droogmans, Louis, Versées, Wim
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6049504/
https://ncbi.nlm.nih.gov/pubmed/29848639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.064345.117
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