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Limitations of alignment-free tools in total RNA-seq quantification

BACKGROUND: Alignment-free RNA quantification tools have significantly increased the speed of RNA-seq analysis. However, it is unclear whether these state-of-the-art RNA-seq analysis pipelines can quantify small RNAs as accurately as they do with long RNAs in the context of total RNA quantification....

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Détails bibliographiques
Publié dans:BMC Genomics
Auteurs principaux: Wu, Douglas C., Yao, Jun, Ho, Kevin S., Lambowitz, Alan M., Wilke, Claus O.
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2018
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6042521/
https://ncbi.nlm.nih.gov/pubmed/29969991
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-018-4869-5
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