Sailfish enables alignment-free isoform quantification from RNA-seq reads using lightweight algorithms

We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approach...

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Detalhes bibliográficos
Main Authors: Patro, Rob, Mount, Stephen M., Kingsford, Carl
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4077321/
https://ncbi.nlm.nih.gov/pubmed/24752080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2862
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https://ncbi.nlm.nih.gov/pmc/articles/PMC4077321/
https://ncbi.nlm.nih.gov/pubmed/24752080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2862