Molecular detection of human papillomavirus (HPV) in highly fragmented DNA from cervical cancer biopsies using double-nested PCR

Archived Formalin-Fixed Paraffin-Embedded (FFPE) tissue specimens can be a valuable source of human papillomavirus (HPV) nucleic acids for molecular biological analyses in retrospective studies. Although successful amplification with polymerase chain reaction (PCR) is essential for analysis of HPV D...

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Bibliographic Details
Published in:MethodsX
Main Authors: Tawe, Leabaneng, Grover, Surbhi, Narasimhamurthy, Mohan, Moyo, Sikhulile, Gaseitsiwe, Simani, Kasvosve, Ishmael, Paganotti, Giacomo M.
Format: Artigo
Language:Inglês
Published: Elsevier 2018
Subjects:
Biochemistry, Genetics and Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6035908/
https://ncbi.nlm.nih.gov/pubmed/29992095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2018.05.018
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