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A Caspase-3 Reporter for Fluorescence Lifetime Imaging of Single-Cell Apoptosis

Fluorescence lifetime imaging (FLIM) is a powerful imaging modality used to gather fluorescent reporter data independent of intracellular reporter intensity or imaging depth. We applied this technique to image real-time activation of a reporter for the proteolytic enzyme, caspase-3, in response to a...

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Detalhes bibliográficos
Publicado no:Cells
Main Authors: Buschhaus, Johanna M., Humphries, Brock, Luker, Kathryn E., Luker, Gary D.
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6025355/
https://ncbi.nlm.nih.gov/pubmed/30720785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells7060057
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