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New chemically reactive dsDNAs containing single internucleotide monophosphoryldithio links: reactivity of 5′-mercapto-oligodeoxyribonucleotides

Novel modified DNA duplexes with single bridging 5′-SS-monophosphoryldithio links [-OP(=O)-O(–)-SS-CH(2)-] were synthesized by autoligation of an oligonucleotide 3′-phosphorothioate and a 5′-mercapto-oligonucleotide previously converted to a 2-pyridyldisulfide adduct. Monophosphoryldisulfide link fo...

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Udgivet i:Nucleic Acids Res
Principais autores: Metelev, Valeri G., Borisova, Oxana A., Volkov, Eugene M., Oretskaya, Tatiana S., Dolinnaya, Nina G.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2001
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Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC60246/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11574689/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.19.4062
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