An optimized protocol for the extraction and quantification of cytosolic DNA in mammalian cells

Summary: Leakage of mitochondrial or nuclear DNA into the cytosol can occur following viral infections, radiation damage, and some cancers. Here, we present an optimized protocol for isolating and quantifying cytosolic DNA from mammalian cells. We describe steps for collecting cytosolic fractions fr...

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Autors principals:	Aminu S. Jahun, Frederic Sorgeloos, Ian G. Goodfellow
Format:	Artigo
Idioma:	Inglês
Publicat:	Elsevier 2024-03-01
Col·lecció:	STAR Protocols
Matèries:	Cancer Cell Biology Cell separation/fractionation Cell-based Assays Immunology Molecular Biology
Accés en línia:	http://www.sciencedirect.com/science/article/pii/S2666166724000789
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