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Duplex Scorpion primers in SNP analysis and FRET applications

Scorpions are fluorogenic PCR primers with a probe element attached at the 5′-end via a PCR stopper. They are used in real-time amplicon-specific detection of PCR products in homogeneous solution. Two different formats are possible, the ‘stem–loop’ format and the ‘duplex’ format. In both cases the p...

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Dades bibliogràfiques
Publicat a:Nucleic Acids Res
Autors principals: Solinas, Antonio, Brown, Lynda J., McKeen, Catherine, Mellor, John M., Nicol, Jamie, Thelwell, Nicky, Brown, Tom
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2001
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC60224/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11600715/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.20.e96
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