Duplex Scorpion primers in SNP analysis and FRET applications

Scorpions are fluorogenic PCR primers with a probe element attached at the 5′-end via a PCR stopper. They are used in real-time amplicon-specific detection of PCR products in homogeneous solution. Two different formats are possible, the ‘stem–loop’ format and the ‘duplex’ format. In both cases the p...

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書誌詳細

出版年:	Nucleic Acids Res
主要な著者:	Solinas, Antonio, Brown, Lynda J., McKeen, Catherine, Mellor, John M., Nicol, Jamie, Thelwell, Nicky, Brown, Tom
フォーマット:	Artigo
言語:	Inglês
出版事項:	Oxford University Press 2001
主題:	NAR Methods Online
オンライン･アクセス:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC60224/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11600715/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.20.e96
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