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Critical comparison of FRET-sensor functionality in the cytosol and endoplasmic reticulum and implications for quantification of ions

Genetically-encoded sensors based on fluorescence resonance energy transfer (FRET) are powerful tools for quantifying and visualizing analytes in living cells, and when targeted to organelles have the potential to define distribution of analytes in different parts of the cell. However, quantitative...

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Detalhes bibliográficos
Publicado no:Anal Chem
Main Authors: Carter, Kyle P., Carpenter, Margaret C., Fiedler, Brett, Jimenez, Ralph, Palmer, Amy E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6018025/
https://ncbi.nlm.nih.gov/pubmed/28758723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.7b02933
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