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Minimizing ATP depletion by oxygen scavengers for single-molecule fluorescence imaging in live cells
The stability of organic dyes against photobleaching is critical in single-molecule tracking and localization microscopy. Since oxygen accelerates photobleaching of most organic dyes, glucose oxidase is commonly used to slow dye photobleaching by depleting oxygen. As demonstrated here, pyranose-2-ox...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6016803/ https://ncbi.nlm.nih.gov/pubmed/29866842 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1717724115 |
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