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Visualizing recycling synaptic vesicles in hippocampal neurons by FM 1-43 photoconversion

Exo–endocytotic turnover of synaptic vesicles (SVs) at synapses between hippocampal neurons in culture was examined by electron microscopy (EM). We carried out photoconversion (PC) of the fluorescent endocytotic marker FM 1-43 by using 3,3′-diaminobenzidine to convert the dye signal into an electron...

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Bibliografische gegevens
Hoofdauteurs: Harata, Nobutoshi, Ryan, Timothy A., Smith, Stephen J, Buchanan, JoAnn, Tsien, Richard W.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The National Academy of Sciences 2001
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC60125/
https://ncbi.nlm.nih.gov/pubmed/11675506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.171442798
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