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Visualizing recycling synaptic vesicles in hippocampal neurons by FM 1-43 photoconversion

Exo–endocytotic turnover of synaptic vesicles (SVs) at synapses between hippocampal neurons in culture was examined by electron microscopy (EM). We carried out photoconversion (PC) of the fluorescent endocytotic marker FM 1-43 by using 3,3′-diaminobenzidine to convert the dye signal into an electron...

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Detalhes bibliográficos
Main Authors: Harata, Nobutoshi, Ryan, Timothy A., Smith, Stephen J, Buchanan, JoAnn, Tsien, Richard W.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC60125/
https://ncbi.nlm.nih.gov/pubmed/11675506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.171442798
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