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Labeling and identifying cell-type-specific proteomes in the mouse brain
We develop an approach to tag proteomes synthesized by specific cell-types in dissociated cortex, brain slices, and the brain of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase/tRNA(XXX) pair in a cell type of interest and providing a non-canonical amino acid with...
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| Publicado no: | Nat Biotechnol |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6010150/ https://ncbi.nlm.nih.gov/pubmed/29251727 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.4056 |
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