Experimental and bioinformatic approach to identifying antigenic epitopes in human α- and β-enolases

Human α- and β-enolases are highly homologous enzymes, difficult to differentiate immunologically. In this work, we describe production, purification and properties of anti-α- and anti-β-enolase polyclonal antibodies. To raise antibodies, rabbits were injected with enolase isoenzymes that were purif...

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Detalhes bibliográficos
Publicado no:Biochem Biophys Rep
Principais autores: Pietkiewicz, Jadwiga, Danielewicz, Regina, Bednarz-Misa, Iwona S., Ceremuga, Ireneusz, Wiśniewski, Jerzy, Mierzchala-Pasierb, Magdalena, Bronowicka-Szydełko, Agnieszka, Ziomek, Edmund, Gamian, Andrzej
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2018
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6005794/
https://ncbi.nlm.nih.gov/pubmed/29922723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2018.05.008
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