Structure-Function Analysis of Chloroplast Proteins via Random Mutagenesis Using Error-Prone PCR

Site-directed mutagenesis of chloroplast genes was developed three decades ago and has greatly advanced the field of photosynthesis research. Here, we describe a new approach for generating random chloroplast gene mutants that combines error-prone polymerase chain reaction of a gene of interest with...

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Bibliographic Details
Published in:Plant Physiol
Main Authors: Dumas, Louis, Zito, Francesca, Auroy, Pascaline, Johnson, Xenie, Peltier, Gilles, Alric, Jean
Format: Artigo
Language:Inglês
Published: American Society of Plant Biologists 2018
Subjects:
Breakthrough Technologies
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6001340/
https://ncbi.nlm.nih.gov/pubmed/29703866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.17.01618
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