Addition of Exogenous γ-Glutamyl Hydrolase Eliminates the Need for Lengthy Incubation of Whole-Blood Lysate for Quantitation of Folate Vitamers by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

BACKGROUND: Measurement of folate monoglutamates by HPLC–tandem mass spectrometry (HPLC-MS/MS) in whole-blood lysate (WBL) requires lengthy incubation before analysis, risking degradation of labile folate vitamers. OBJECTIVE: We explored whether the addition of a commercially available recombinant e...

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Publicado en:Curr Dev Nutr
Autores principales: Stamm, Rosemary A, Fazili, Zia, Pfeiffer, Christine M
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2017
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Original Research
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5998367/
https://ncbi.nlm.nih.gov/pubmed/29955722
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/cdn/nzx003
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