Profiling the tumor microenvironment proteome in prostate cancer using laser capture microdissection coupled to LC⿿MS⿿A technical report

Laser capture microdissection (LCM) allows microscopic procurement of specific cell types from tissue sections. Here, we present an optimized workflow for coupling LCM to LC⿿MS/MS including: sectioning of tissue, a standard LCM workflow, protein digestion and advanced LC⿿MS/MS. Soluble proteins extr...

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Bibliografske podrobnosti
izdano v:EuPA Open Proteom
Main Authors: Staunton, L., Tonry, C., Lis, R., Finn, S., O⿿Leary, J., Loda, M., Bowden, M., Pennington, S.R.
Format: Artigo
Jezik:Inglês
Izdano: Elsevier 2015
Teme:
Special Section: Proceedings of the 10th Siena Meeting "From Genome to Proteome - 20 years of Proteomics" (Aug 31 - Sep 4, 2014, Siena, Italy)
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5988569/
https://ncbi.nlm.nih.gov/pubmed/29900095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.euprot.2015.11.001
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