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Proteomic Analysis of the Cell Cycle of Procylic Form Trypanosoma brucei
We describe a single-step centrifugal elutriation method to produce synchronous Gap1 (G1)-phase procyclic trypanosomes at a scale amenable for proteomic analysis of the cell cycle. Using ten-plex tandem mass tag (TMT) labeling and mass spectrometry (MS)-based proteomics technology, the expression le...
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| Publicado en: | Mol Cell Proteomics |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
The American Society for Biochemistry and Molecular Biology
2018
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5986242/ https://ncbi.nlm.nih.gov/pubmed/29555687 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.RA118.000650 |
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