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Human Induced Pluripotent Stem Cell Production and Expansion from Blood using a Non-Integrating Viral Reprogramming Vector
We describe a method to transform blood lymphocytes into human induced pluripotent stem cells by delivering 4 transcription factors with a non-integrative virus. Using human peripheral blood mononuclear cells (PBMCs) as the source cell type for hiPSC reprogramming is advantageous since blood samples...
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| Publié dans: | Curr Protoc Mol Biol |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5986089/ https://ncbi.nlm.nih.gov/pubmed/29851250 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.58 |
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