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A systematic analysis of the expression of the anti-HIV VRC01 antibody in Pichia pastoris through signal peptide optimization

Pichia pastoris (Komagataella phaffi) has been used for recombinant protein production for over 30 years with over 5000 proteins reported to date. However, yields of antibody are generally low. We have evaluated the effect of secretion signal peptides on the production of a broadly neutralizing anti...

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Detalles Bibliográficos
Publicado en:Protein Expr Purif
Main Authors: Aw, Rochelle, McKay, Paul F., Shattock, Robin J., Polizzi, Karen M.
Formato: Artigo
Idioma:Inglês
Publicado: Academic Press 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5982643/
https://ncbi.nlm.nih.gov/pubmed/29601964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2018.03.013
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