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Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris
BACKGROUND: Multiple cognate gene copy clones have often been used in order to increase the yield of recombinant protein expression in the yeast Pichia pastoris. The method of posttransformational vector amplification (PTVA) has allowed for the efficient generation of multi-copy clones in P. pastori...
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| Veröffentlicht in: | Microb Cell Fact |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4744420/ https://ncbi.nlm.nih.gov/pubmed/26849882 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0432-8 |
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