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Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay

The ability to perform in vitro splicing assays has paved the way for in-depth studies of the mechanisms and machinery involved in the process of splicing. The in vitro splicing assay is a valuable experimental approach that combines the complexity of the spliceosome and regulatory systems with the...

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Vydáno v:Methods Mol Biol
Hlavní autoři: Movassat, Maliheh, Shenasa, Hossein, Hertel, Klemens J.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5972008/
https://ncbi.nlm.nih.gov/pubmed/28766286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7204-3_2
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