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Preparation of Splicing Competent Nuclear Extracts

Splicing components play an essential role in mediating accurate and efficient splicing. The complexity of the spliceosome and its regulatory networks increase the difficulty of studying the splicing reaction in detail. Nuclear extracts derived from HeLa cells provide all of the obligatory component...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Webb, Chiu-Ho T., Hertel, Klemens J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4501576/
https://ncbi.nlm.nih.gov/pubmed/24549659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-980-2_8
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