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Preparation of Splicing Competent Nuclear Extracts

Splicing components play an essential role in mediating accurate and efficient splicing. The complexity of the spliceosome and its regulatory networks increase the difficulty of studying the splicing reaction in detail. Nuclear extracts derived from HeLa cells provide all of the obligatory component...

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Bibliografiske detaljer
Udgivet i:Methods Mol Biol
Main Authors: Webb, Chiu-Ho T., Hertel, Klemens J.
Format: Artigo
Sprog:Inglês
Udgivet: 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4501576/
https://ncbi.nlm.nih.gov/pubmed/24549659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-980-2_8
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