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Stopped-flow fluorometric ion flux assay for ligand-gated ion channel studies

Quantitative investigations into functional properties of purified ion channel proteins using standard electrophysiological methods is challenging, in particular for the determination of average ion channel behavior following rapid changes in experimental conditions (e.g., ligand concentration). Her...

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Détails bibliographiques
Publié dans:Methods Mol Biol
Auteurs principaux: Posson, David J., Rusinova, Radda, Andersen, Olaf S., Nimigean, Crina M.
Format: Artigo
Langue:Inglês
Publié: 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5971093/
https://ncbi.nlm.nih.gov/pubmed/29058195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7362-0_17
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