Simple discrimination of sub-cycling cells by propidium iodide flow cytometric assay in Jurkat cell samples with extensive DNA fragmentation

Human leukemia Jurkat T cells were analyzed for apoptosis and cell cycle by flow cytometry, using the Annexin V/propidium iodide (PI) standard assay, and a simple PI staining in Triton X-100/digitonin-enriched PI/RNase buffer, respectively. Cells treated with doxorubicin or menadione displayed a ver...

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Pubblicato in:Cell Cycle
Autori principali: Babes, Ramona Madalina, Tofolean, Ioana Teodora, Sandu, Roxana Gabriela, Baran, Oana Elena, Cosoreanu, Vlad, Ilie, Maria Teodora, Duta, Alexandru Ionut, Ceausescu, Maria Catalina, Ciucur, Paul Mihai, Costache, Simona, Ganea, Constanta, Baran, Irina
Natura: Artigo
Lingua:Inglês
Pubblicazione: Taylor & Francis 2018
Soggetti:
Reports
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5969556/
https://ncbi.nlm.nih.gov/pubmed/29417873
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15384101.2018.1426415
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