Optimizing FRET-FLIM Labeling Conditions to Detect Nuclear Protein Interactions at Native Expression Levels in Living Arabidopsis Roots

Protein complex formation has been extensively studied using Förster resonance energy transfer (FRET) measured by Fluorescence Lifetime Imaging Microscopy (FLIM). However, implementing this technology to detect protein interactions in living multicellular organism at single-cell resolution and under...

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Detalhes bibliográficos
Publicado no:Front Plant Sci
Main Authors: Long, Yuchen, Stahl, Yvonne, Weidtkamp-Peters, Stefanie, Smet, Wouter, Du, Yujuan, Gadella, Theodorus W. J., Goedhart, Joachim, Scheres, Ben, Blilou, Ikram
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2018
Assuntos:
Plant Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5962846/
https://ncbi.nlm.nih.gov/pubmed/29868092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2018.00639
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