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Optimizing FRET-FLIM Labeling Conditions to Detect Nuclear Protein Interactions at Native Expression Levels in Living Arabidopsis Roots

Protein complex formation has been extensively studied using Förster resonance energy transfer (FRET) measured by Fluorescence Lifetime Imaging Microscopy (FLIM). However, implementing this technology to detect protein interactions in living multicellular organism at single-cell resolution and under...

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Publicat a:Front Plant Sci
Autors principals: Long, Yuchen, Stahl, Yvonne, Weidtkamp-Peters, Stefanie, Smet, Wouter, Du, Yujuan, Gadella, Theodorus W. J., Goedhart, Joachim, Scheres, Ben, Blilou, Ikram
Format: Artigo
Idioma:Inglês
Publicat: Frontiers Media S.A. 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5962846/
https://ncbi.nlm.nih.gov/pubmed/29868092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2018.00639
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