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Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples

Nucleic acid material of adequate quality is crucial for successful high-throughput sequencing (HTS) analysis. DNA and RNA isolated from archival FFPE material are frequently degraded and not readily amplifiable due to chemical damage introduced during fixation. To identify optimal nucleic acid extr...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Kresse, Stine H., Namløs, Heidi M., Lorenz, Susanne, Berner, Jeanne-Marie, Myklebost, Ola, Bjerkehagen, Bodil, Meza-Zepeda, Leonardo A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5957415/
https://ncbi.nlm.nih.gov/pubmed/29771965
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0197456
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