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Precision Tagging: A Novel Seamless Protein Tagging by Combinational Use of Type II and Type IIS Restriction Endonucleases

Protein tagging is a powerful tool for performing comprehensive analyses of the biological functions of a protein of interest owing to the existence of a wide variety of tags. It becomes indispensable in some cases, such as in tracking protein dynamics in a live cell or adding a peptide epitope due...

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Bibliographische Detailangaben
Veröffentlicht in:Bio Protoc
Hauptverfasser: Xu, Zhen, Rui, Yan-Ning, Hagan, John P., Kim, Dong H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5951406/
https://ncbi.nlm.nih.gov/pubmed/29770347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2721
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