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Seamless Gene Tagging by Endonuclease-Driven Homologous Recombination

Gene tagging facilitates systematic genomic and proteomic analyses but chromosomal tagging typically disrupts gene regulatory sequences. Here we describe a seamless gene tagging approach that preserves endogenous gene regulation and is potentially applicable in any species with efficient DNA double-...

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Autores principales: Khmelinskii, Anton, Meurer, Matthias, Duishoev, Nurlanbek, Delhomme, Nicolas, Knop, Michael
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2011
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3161820/
https://ncbi.nlm.nih.gov/pubmed/21915245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0023794
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